The Nest Group, Inc., Applications and Protocols Index
Related Links:
using ion exchange resins
&,& an on-line book by Dr. Yuri Kazakevich and Dr. Harold McNair
Adam P. Schellinger and Peter W. Carr, LCGC, JUNE (): 544-48. Useful for RPC and/or HILIC mobile phase composition selection.
: Use of 100% water with TARGA C18 increases retention of polar molecules for quantitative experiments.
Eliminate tailing
due to metals chelating
sample on frits or MS emitter tips with a 50mM disodium EDTA treatment (an off-line, non-volatile treatment), or more conveniently, with acetylacetonate in the mobile phase.
for Either Desalting or Trapping of Polar Solutes on Trap Columns.
when using Superficially Porous Packings: If not fast enouogh, at low concentrations, narrow peaks appear to give non-linear responses.
An Introduction to HILIC Chromatography, A Tutorial and Applications Manual:
An Introduction to Ion Chromatography and trouble shooting manual:
mixtures [(H-0): Bosch et al., Anal. Chem. 68 (-3657]:
. [(H-00): Canals I, JA Portal, E Bosch, and M Roses
Retention of ionizable compounds on HPLC. 4. Mobile-phase pH measurement in methanol/water
Anal Chem, April 15, 2000;
procedures are evaluated: - measurement of the pH of the aqueous HPLC buffer before mixing it with
the organic modifier, - measurement of the pH of the HPLC buffer after mixing it with the organic
modifier using a pH electrode system calibrated with aqueous buffers, and
- measurement of the pH
of the HPLC buffer after mixing it with the organic modifier but calibrating the electrode system
with reference buffers prepared in the same mixed solvent used as mobile phase.
Following IUPAC
definitions and recommendations, the three pH values are related to the pH scales: w(w)pH, s(w)pH, and s(s)pH,
respectively. The relationships between these three pH scales are also presented. The retention of
several compounds with acid/base behavior in a C-18 and a polymeric column with buffered methanol/water
as mobile phase is related to the mobile phase pH value measured in the three pH scales.
They demonstrate that the s(w)pH and s(s)pH scales give better relationships than the w(w)pH scale
(pH measured in the aqueous buffer before mixing it with the organic modifier), commonly used on HPLC.
The s(w)pH scale is especially recommended because of its simplicity of measurement: the pH is
measured after mixing the aqueous buffer with the organic modifier, but the pH calibration is performed
with the common aqueous reference buffers.
The Interpretation of pH in Partially Aqueous Mobile Phases.& A pH change occurs when organic solvent is present and how silica columns can be used at a higher pH.
Buffer Selection and Capacity.& Selecting buffers for use in partially aqueous mobile phases.
Preparation of Buffers.& The importance of using the weights-and-volumes format.
Monitoring the effects of
on selectivity for ionic HILIC phases with.
Molarity of Concentrated Reagents
to make 1 Molar Solutions of Acids & Bases for LC-MS or Ion Exchange
for ZIC-HILIC columns.
Data Tables for Column Dimension Changes.
Size Exclusion:
Barth, H.G. and Saunders, G.D., "Fundamentals and Properties of Size-Exclusion Chromatography Packings and Columns", Supplement to LCGC, Recent Developments in LC Column Technology, April .
Solid Phase Extraction:
(adapted, with thanks to Prof. Michael Burke, Univ. AZ) for robust results.
for LCMS or ELSD systems.
Volume Dependency of Retention with Partition Columns:
: Use of 100% water with TARGA C18 increases retention of polar molecules for quantitative experiments.
Go back to .
HPLC Column Care
HIC for Proteins:
Hydrophobic Intereaction Technical Sheet
shooting HYDROCELL& polymeric HIC columns.
for HIC and IEX.
HILIC for Polar Differences in
Molecules:
& Aspartamide Technical Sheet ()
(for Complex Carbohydrates) Technical Sheet ()
ZIC&-HILIC & ZIC&-pHILIC General Operational Parameters Before Getting Started:
Analytical Columns Technical Sheet
Columns Technical Sheet
Polymeric Columns Technical Sheet
Capillary Columns Technical Sheet
An Introduction to HILIC Chromatography, A Tutorial and Applications Manual:
Ion Exchange:
Strong Cation Exchange Technical Sheet
& Weak Cation Exchange Technical Sheet
Weak Anion Exchange, Technical Sheet
PolyCAT A - PolyWAX LP, Mixed-Bed Ion Exchange Technical Sheet
shooting HYDROCELL& polymeric IEX columns.
for IEX and HIC.
Nano-flow Capillary :
with Higgins Ca&llary& columns.
to reduce nano-flow chromatographic system dead volume in tubing connections
Reversed Phase:
Information
Higgins Ca&llary& column
Higgins & Vydac Column
Cartridges to the Vydac &...GD54& Guard Cartridges
Size Exclusion (SEC):
PolyHYDROXYETHYL Aspartamide Technical Sheet ()
SPE Column Operating :
Go back to .
Small Molecule and Combi-Chem SPE Separations
Cerex Filtration/Drying Manifolds & SPE Cartridges for Purificaton and Recovery
for positive pressure SPE filtration or drying
performance
(small particle) SPE. Small particle SPE means higher speed, higher loading, and smaller elution volume SPE.
TLC to Flash:
TLC to Flash:
TLC to Flash:
TLC to Flash:
Part Numbers and Prices
and MicroTip micro-SPE & Desalting Kits:
columns and & for microliter sample prep. Concentrate, desalt
or fractionate 2 - 400&l of chemical or biological materials
on over 20 RPC, IEX, and Polar micro-SPE chemistries or 2 - 150&l on SEC materials.
Part Numbers and Prices
for ERLIC, HILIC, RPC, SCX, SEC, WAX, or WCX MiniSpin products.
columns, the tips equivalent to the UltraMicroSpin columns for microliter sample prep. Concentrate, desalt or
fractionate 5 - 100&l (3-30 &g) of chemical or biological materials on over
20 RPC, IEX, and Polar chemistries
Part Numbers and Prices
Micro filter &
empty columns remove the particulates from in-gel digests to prolong capillary LC-MS column life.
with caps to remove solid reactants from micro quantities of samples. See: Xia B., et al. Anal Biochem. 2009 Apr 15;387(2):162-70. Epub 2009 Feb 10.
Removal of phospholipids using HILIC on silica SPE cartridges with
for eliminating matrix effects in the analysis of biological fluids when using RPC LC-MS.
Volume Dependent Results for Retention with Tip Columns for MS &-SPE:
. Use TARGA C18 to increase retention of polar molecules for quantitative experiments, or change loading solvent composition to increase retention.
for Robust Solid Phase Extraction Results.
Ultra-Micro Spin RP clean-up for
or HILIC for
removal from
protein digests. Useful for MS applications, due to higher capacity than ZipTip& C18.
Part Numbers and Prices
Go back to .
Small Molecule and Combi-Chem HPLC Separations
Higgins Analytical& HPLC columns
(Exceptionally Good Performance, A Wide Range of Configurations.) Our &Best Value& Product Line.
(3.67 MB pdf) 0.075 - 22mmID columns.
Catalogs of HAISIL& HPLC Columns
0.075 - 0.15mmID Nano-Capillaries
0.32 - 0.5mmID microCapillaries
Short, Direct Connect, 2.1mmID Narrowbore
0.3-1.0mmID Microbore Guard/Trap
(Type A Silica) HPLC Columns
Kromasil& 100 small pore columns.
of Kromasil& to PHALANX& for pharmaceutical method equivalency.
(Type B Silica) HPLC Columns
HAISIL RS,
C4 & C18 for Peptide applications.
Comparison of PROTO& 200 to & C18 Peptide Columns.
Comparison of PROTO 200 for .
HAISIL RS,
C4 & C18 for Protein applications.
HAISIL TS, & C8 & C18 for Polar Analytes & Proteomics.
HAISIL CS, & Silica, CN, Phenyl, C8, & C18 phases, excellent for Small Molecule applications.
Heavily Coated (Type B Silica) Phases:
HAISIL VS, & Ultra-high density coating for wide pH range applications.
HAISIL HL,
High density coating for Combi-Chem & Fast LC Analyses.
HAISIL PS, & Water-wettable, Ultra-high density coating for wide pH range applications.
for HAISIL Columns:
on HAISIL Columns:
(low organic, 100% water wettable RPC) for DABSYL or AccQ-Tag& derivatives
for fast PTH analyses
HAISIL PTC
PTC analyses
for Pico-Tag& derivatives
Amines (without tailing), or for LC-MS/MS, &no TFA&
peptide separations:
with WOW! selectivity in C8, C18, CN or Phenyl phases that do not
require base deactivation.
Compound analyses without buffer
analyses of small molecules on Heavily Loaded HAISIL columns
Metabolomics & Proteomics:
Fast equilibrating & 100% aqueous loading
extremely wide polarity range separations of
Comparison of TARGA to BetaBasic& for
C4 & C18 Peptide Columns
Comparison of & C18 to Other Columns for Phosphopeptides.
C4 & C18 Protein Columns
Comparison of & C4 to Vydac& C4 for proteins.
Comparison of PROTO&300 C4 to Vydac C8 and Zorbax& C8 for .
HPLC Cartridge Columns.
Part Numbers and Prices
Go back to .
MACCEL& HPLC Columns
Enhanced polar selectivity on MACCEL AQPS&, a water wettable (low organic RPC) phase, with great selectivity for
all polar molecules.
: Polar Selectivity of unbuffered acids and bases on AQPS phases.
of acids and bases on AQPS phases under acidic buffered, ACN conditions.
for acids and bases on AQPS phases: neutral buffered, MeOH conditions.
Compare selectivity on MACCEL Spheribond& ODS1 and ODS2 to Waters& Spherisorb& ODS1 and ODS2
: Selectivity is similar under a number of comparative conditions.
: Selectivity is very close under a number of comparative conditions.
Polar molecule selectivity on MACCEL Hypersorb& ODS is very close to Thermo& Hypersil& ODS
: Selectivity under a number of comparative conditions is very similar.
Maccel Analytical and Microbore HPLC columns
MACCEL Small Molecule HPLC
Grace/Vydac& Small Molecule HPLC Columns
Go back to .
Monolithic Columns for Small Molecules & Combi-Chem
EMD Chemicals&
columns catalog. Low back pressure, faster assays, longer life columns.
Chromolith&
Go back to .
HPLC Columns for Protein & Peptide Analysis Applications:
Detergent Removal
Technical Sheet for HILIC microSPE
Cartridges or SDS Removal Guard columns.
Peptide Retention Time Calculations
Oleg Krokhin's . New sequence-specific
correction factors for prediction of peptide retention in RP-HPLC: application to
protein identification by off-line HPLC-MALDI-MS. This version has been developed for the same set of ~2000
tryptic peptides and features new correction factors related to a peptide's propensity to form helical
structures. Correlation about 0.98 (R-squared value) was obtained for the training set of 2000 peptides
compared to 0.96 for version 2.
Higgins Analytical HILIC and RPC Peptide Columns
(3,468K pdf) 0.075 - 22mmID columns.
Catalogs of HAISIL& HPLC Columns
0.075 - 0.15mmID Nano-Capillaries
0.32 - 0.5mmID microCapillaries
Short, Direct Connect, 2.1mmID Narrowbore
0.3-1.0mmID Microbore Guard/Trap
HAISIL& 100 Silica Columns
for HAISIL HILIC & Peptide and Protein Columns:
on HAISIL Columns:
(low organic, 100% water wettable RPC) for DABSYL or AccQ-Tag& derivatives
for fast PTH analyses
HAISIL PTC
PTC analyses
for Pico-Tag& derivatives
RPC columns are excellent for
smaller peptide, &no TFA& separations
(without tailing).
Metabolomics & Proteomics:
Fast equilibrating & 100% aqueous loading
extremely wide polarity range separations of
Comparison of TARGA to BetaBasic& for .
deacylase activity: Using short TARGA, Sprite columns to monitor a NAD-Dependent Protein Lysine Demalonylase and Desuccinylase.
Water-wettable, Ultra-high density coating for a wider pH range for peptide applications.
C4 & C18, Peptide columns .
Comparison of PROTEO 200 to & Peptide Columns.
Comparison of PROTO 200 for .
Greater capacity of PROTO 200 C4, 200&A compared to 300&A, C18 for peptide .
Protein columns.
Comparison of PROTO300 C4 for
Comparison of
C4 to Vydac C4 for proteins.
Comparison of PROTO300 C4 to Vydac C8 and Zorbax& C8 for .
PROTO300 C4 for .
Water-wettable, 10x faster RPC equilibration
using 100% aqueous conditions, where other AQ columns need 2-5% ACN, are
great for LC-MS/MS &no TFA& proteomic capture and separations of peptides using formic acid.
&& HAISIL& Reversed Phase (Type A Silica)
HPLC Columns and Cartridge Products have been used for virus particles and protein, peptide
separations.
Part Numbers and Prices
Go back to .
IEX, HIC, RPC & SEC Columns for Proteins &
Antibody Drug Conjugates
columns, Operation
Mixed-Bed Ion Exchange for Complete
and Fractionation
& Weak Cation Exchange Technical Sheet
for Weak Cation Exchange of Peptides
of Proteins
by WCX. Comparison of 3&m to 5&m columns for FACS & A1c.
Bulletin 2006
Antibody Variant Analysis Application Sheet
Application Sheet
SCX Strong Cation Exchange Spec Sheet
fractionation of proteins for
Use of pH and salt gradients for 2-D
proteomics fractionation
of tryptic digests.
HILIC-SCX of
from excess lipids.
Weak Anion
Exchange, Spec Sheet
Dropbox of
ERLIC-WAX, Tryptic
(ASMS poster)
Phosphopeptide SPE Instructions
(Hydrophobic Interaction)
its implications for HIC. A discussion of the ranking of various ions toward their ability to
retain (precipitate) a mixture of proteins. See also, Douglas J. Tobias and John C. Hemminger, Science, 319, 08) for a discussion of Specific Ion Effects regarding the hydration of ions on surfaces.
HIC Analysis
HIC Spec Sheet
and Methods
Development.
for Complex Carbohydrate Separations, Spec Sheet
Rapid, Sensitive Combinatorial Library Screening: 10nM
Screening by SEC
PolyHYDROXYETHYL Aspartamide Technical Sheet
of PolyLC column references.
Part Numbers and Prices
Go back to .
Sepax-Technologies&
IEX, RPC & SEC Columns for Biomolecules &
Antibody Drug Conjugates
Barth, H.G. and Saunders, G.D., , Supplement to LCGC, Recent Developments in LC Column Technology, April . A theoretical basis for SEC, which helps explain why Sepax SEC columns are so effective.
Comparison of
IEX Resins for Intact Proteins
Analysis using 1000&A RPC, PolyRP&-1000.
: Analyses using Zenix SEC & WCX NP5 columns.
Using Zenix& SEC Columns
Analysis Using Zenix& SEC-300 Columns
in the Presence of Tween Detergents Using Sepax SEC Columns
for Biomolecules.
for Biomolecules.
(lay-down) SEC Phases Provide a Wider SEC Range of Separation for Hydrophobic Biomolecules.
Go back to .
Grace/Vydac&
for Vydac columns
Vydac TP (polymeric bonded phase) columns for peptides
Comparison of 218TP to
C18 Peptide Columns for 20-30 amino acid peptides.
Comparison of & C4 to Vydac C4 for proteins.
Everest& C18 (monomeric) Ultra High Resolution Protein & Peptide Tryptic Mapping Columns.
Comparison using
to 100&A (LC Packings) column.
Comparison to & 200 C18 Peptide Columns for Phosphopeptides.
Importance of
for a reproducible peptide separation.
Mobile Phase
effects on
MS bonded silica columns
Proteomic applications on &low TFA& MS media in prepacked
from 0.075mmID - 4.6mmID.
HPLC separations (TFA, PO4, HOAc, HCl) Spec. Sheet
Part Numbers and Prices
Go back to .
HILIC Methods for Separating
Polar Molecules by Polar Differences - Alt.: &Aqueous Normal Phase& (ANP)
(underivatized) from blood for tissue transplant rejection monitoring
(underivatized) HILIC LC-MS/MS from plant extracts
Metabolites
Dropbox of .
P. Hao, et al. &Enhanced Separation and Characterization of Deamidated Peptides
with RP-ERLIC-Based Multidimensional Chromatography Coupled
with Tandem Mass Spectrometry&, dx.doi.org/10.1021/pr201048c | J. Proteome Res. 04-1811.
(aminoglycoside) antibiotics with LC-MS Detection.
analysis in food
HILIC Comparison to RPC ().
Analysis with Volatile Buffers.
Analysis using Electrostatic Repulsion Hydrophilic Interaction (ERLIC) [alt. eHILIC].
pH Effects in HILIC
eHILIC assay with
LC-MS or ELSD detection
Phospholipid removal using HILIC on silica SPE cartridges with
for eliminating matrix effects in the analysis of biological fluids when using RPC LC-MS.
of Histone H1.1 HILIC-CEX
Isolation using Electrostatic Repulsion Hydrophilic Interaction (ERLIC) [alt. e-HILIC].
Polar Molecules
(urea, allantoin, lysidone, amino adipic acid, pipicolic acid) applications
HILIC Column Comparisons
Dropbox of .
Comparison of PolyHYDROXYETHYL A& to &
for small molecule HILIC LC-MS analysis of fermentation broth.
HILIC for small polar . A Comparison (Y. Guo & S. Gaiki, J. Chromatogr. A, ) 71-80.) of
functional groups.
HILIC, even on raw silica, .
LCGC article: & Hydrophilic Interaction Chromatography Using Silica Columns for the Retention of Polar Analytes
[small molecules sic] and Enhanced ESI-MS Sensitivity&
for HILIC MiniSpin products.
columns (tips equivalent to the UltraMicroSpin columns for microliter sample prep).
& Aspartamide HILIC Technical Sheet
HILIC Articles
(alt: &Aqueous Normal Phase& (ANP)) - HILIC Is Partition Chromatography with Electrostatic (e-HILIC) and Hydrogen Donor Mechanisms Utilized for Separations.
of PolyLC HILIC column references.
Dropbox of .
Complex Carbohydrate
References
Evaluation of an inverted zwitterionic, , cholinate stationary phase for proteomic 2-D identification of more than 20000 unique peptides corresponding to over 3500 proteins.
ZIC&- for complementary selectivity
HILIC Day, June 16,
A.M. - 4:00 P.M. at NJ .
HILIC . Discusses melamine analysis in food products.
HILIC . Discusses methyl malonic acid analysis in serum, plasma and urine plus an ELSD detection method for
choline and inositol.
HILIC . Discusses diethylene glycol analysis in medicine, and suggestions on how to reduce consumption of acetonitrile.
HILIC . Discusses alternatives to ACN for HILIC assays and announces the U.S. HILIC Day prior to the ASMS meeting in Philadelphia.
HILIC . Illustrates increased sensitivity
for Peptide Mapping by HILIC and references Kato, et al.,
publication: &Application of amino acid analysis using hydrophilic interaction liquid chromatography coupled with isotope dilution mass spectrometry for peptide and protein quantification.&
HILIC . Approaches to the
and an invitation to
webinars and podcasts.
: Petrus Hemstrom and Knut Irgum, J. Sep. Sci., 2006, 29, 1784 - 1821. &Hydrophilic Interaction Chromatography.&
, M. Liu, J. Ostovic, E.X. Chen, N. Cauchon,
J. Chromatogr. A, Mar 2009; 1216(12): 2362-70.
for ZIC-HILIC columns.
Go back to .
SeQuant& ZIC&-HILIC Columns
Applications
for Zwitterion HILIC on a
Betain Sulfonate Surface
LCGC AUG-06: Brian W. Pack and Donald S. Risley
Simultaneous Measurement of Anions & Cations:
Poster on use of
bound to silica for Zwitterion HILIC separations
ZIC- HILIC
for Polar Separations
Zwitterion HILIC of , an improved method
Zwitterion HILIC analysis of
Zwitterion HILIC of
and glucuronated metabolites
Zwitterion HILIC of
nerve agent metabolites
Zwitterion HILIC
Zwitterion HILIC of peptides prior to RPC.
for proteomics.
Zwitterion HILIC of
Zwitterion HILIC of
from biological fluids
, cholinate stationary phase for proteomic 2-D identification of more than 20000 unique peptides corresponding to over 3500 proteins.
Applications
for Zwitterion pHILIC on a
Betain Sulfonate Surface
for Adenosine Phosphate Analysis (AMP, cAMP, dAMP, ADP, dATP and ATP)
Using HILIC Conditions with Volatile Buffers (70% ACN, 30% Ammonium Carbonate, pH 8.8, 100 mM).
(AMP, ADP,
ATP, CoA, Acetyl-CoA, and Pantothenate)
Using HILIC Conditions with Volatile Buffers (80% ACN, 20% 10 mM Ammonium Carbonate, 0.2% NH4OH).
for ZIC-HILIC columns.
Part Numbers and Prices
ZIC&-HILIC & ZIC&-pHILIC General Operational Parameters Before Getting Started:
Columns Technical Sheet
Polymeric Columns Technical Sheet
Capillary Columns Technical Sheet
Go back to .
Complex Carbohydrate Separations
Activated Charcoal SPE
and Their Derivatives on
C18 Desalting and Purification of Oligosaccharides
Glycan Reductive Isotope Labeling (GRIL)
remove solid reactants from micro quantities of samples. See: Xia B., et al. Anal Biochem. 2009 Apr 15;387(2):162-70. Epub 2009 Feb 10.
Using HILIC's Electrostatic (e-HILIC) and Hydrogen Donor Mechanisms for Carbohydrate Separations.
Approaches to the
by ZIC&-HILIC.
Comparison of 100&A to 200&A
for the separation of monosaccharides
and neutral oligosaccharides.
Dramatic effect of a 200&A or greater
on the HILIC separation of derivatized sialylated complex glycans.
conditions for
or sulfated carbohydrates.
IP-NPLC [eHILIC]
high mannose
and sialylated glycopeptides. "Identification and Quantification of Glycoproteins Using Ion-Pairing Normal-Phase LC and MS," Wen Ding, Harald Nothaft, Christine M. Szymanski, and John Kelly,
for Carbohydrate HPLC.
Go back to .
Glycomics and Proteomics Applications:
Amino Acid Specific Peptide & Protein Isolation Spin Columns
BioMolecular Technologies&
Glycomic Separation Techniques
Activated Charcoal SPE:
and Their Derivatives on
(2011 ASMS, Siu Kwan Sze Poster, Nanyang Technological University, Singapore).
On The HILIC Separation of Glycans
IP-NPLC [e-HILIC]
high mannose
and sialylated glycopeptides. "Identification and Quantification of Glycoproteins Using Ion-Pairing Normal-Phase LC and MS," Wen Ding, Harald Nothaft, Christine M. Szymanski, and John Kelly,
Comparison of Hydrazide Covalent Chromatography and ERLIC for .
Glycopeptide Separation Using PROTO300 C4 RPC.
HPLC & SPE Proteomic Separation Techniques
Capillary Columns for
Fractionation.
Comparison of SCX, TiO2, HILIC (HEA) and HILIC (WAX) for
segregation and/or fractionation.
Comparison of eHILIC Solvent Conditions Using PolyWAX LP& (column or SPE) for
(2011 ASMS, Siu Kwan Sze Poster, Nanyang Technological University, Singapore).
Comprehensive
fractionation of peptides vs. SCX prior to RPC for proteomics
: &[sic] comprehensive profiling of rat kidney proteome&, P. Hao, et al., Journal of Proteome Research (impact factor: 5.11). 06/):3520-6. DOI:10.1021/pr100037h.
of peptides prior to RPC for proteomics.
SCX pre-columns for & and
fractionation of proteins for proteomics.
Use of pH and salt gradients for 2-D
proteomics fractionation
of tryptic digests.
columns and
for microliter sample prep. For the hardware challenged:
Use MiniSpin Tips or UltraMicroSpin SCX columns to fractionate, then load
into an autosampler for RPC LC-MS/MS
Micro filter &
empty columns remove the particulates from in-gel digests to prolong capillary LC-MS column life
Mixed-Bed Ion Exchange for Complete
and Fractionation
Go back to .
DNA & RNA Oligonucleotide Separations:
Higgins Analytical&
Analysis on Targa C18 5&m 250x4.6mm
column using 15mM ammonium acetate, pH 6.0 with a gradient to 10% MeOH.
Post Transcriptional Modification,
Separations (C. Callie Coombs, University of Cincinnati, Masters Thesis in Chemistry).
Hydrocell&
Protein and Oligonucleotide separations by HIC or IEX on polymer based columns.
Part Numbers and Prices
shooting guide.
Macherey-Nagel&
for DNA, RNA and Oligonucleotides.
Oligonucleotide separations on NS 1000 and
columns in volatile buffers.
Macherey-Nagel Part
Numbers and Prices
PAGE, DEAE technique for Oligonucleotide clean-up for LC-MS applications:
column after PAGE or RPC column separation
for DeOxy RiboNucleoside Analysis
Using HILIC Conditions with Volatile Buffers.
separation
using a PolySULFOETHYL& A, SCX column and phosphate buffer conditions.
for Adenosine Phosphate Analysis (AMP, cAMP, dAMP, ADP, dATP and ATP)
Using HILIC Conditions with Volatile Buffers (70% ACN, 30% Ammonium Carbonate, pH 8.8, 100 mM).
(AMP, ADP,
ATP, CoA, Acetyl-CoA, and Pantothenate)
Using ERILIC Conditions with Volatile Buffers (80% ACN, 20% 10 mM Ammonium Carbonate, 0.2% NH4OH).
Grace Vydac&
Mono-, di- and tri-phosphate Nucleotides on
SAX columns.
Most common & SEC column and ToyoPearl& media
and answers.
& Comparison to &
for small molecule HILIC LC-MS analysis of fermentation broth
Go back to .
Simple Dialysis
Ultra-filtration, Dialysis,
or 96 Well Plates for
Teflon& DIALYZER& for fast, small volume, salt removal (10&l to 5ml)
Micro DispoDIALYZER& for fast
(10&l to 100&l) salt removal. Disposable Dialysers at a fraction of the cost of slide or cassette products.
Micro Dispo-DIALYZER (1-100&L unit) .
DispoDIALYZER&
(1-10ml) Disposable Dialysers.
for ultra-small volume (1-5&L) ultra-filtration of samples.
SpinCon Operating .
Macro DIALYZER for larger volume dialysis
used in conjunction with the & system.
, HPLC effluent buffer exchange & micro dialysis.
from Labelled Proteins in 20 minutes with Minimal Protein Loss.
Dialyzer Part Numbers &
Go back to .
Equilibrium Dialysis:
of Reusable dialyzers with replaceable membranes or disposable equilibrium dialysers for Protein or DNA Binding Studies.
Individual : 75&L disposable kits.
Modular Plate & with individual membranes on 50-200 &L wells. ASME robotic dimensioned plates.
96-Well DispoEquilibrium DIALYZER
Fast Micro-Equilibrium DIALYZER&
Multi-Equilibrium Dialyser
Equilibrium Dialysis
(Note: 5.7M file using Harvard Apparatus p/n, not Nest p/n)
Equilibrium Dialyzer Part Numbers &
Go back to .
and storage
Dialysis Membrane
Dialysis Membrane
Dialysis Membrane
Membrane Part Numbers &
Dialyzer and Equilibrium Dialyzer
of Equilibrium Dialysis Applications
Equilibrium Dialysis
(Note: 5.7M file using Harvard Apparatus p/n, not Nest p/n)
Dialyzer and Equilibrium Dialyzer
Go back to .
Electrophoresis Applications
Agarose for DNA Electrophoresis
General Characteristics & Applications
Agarose: Characteristics,
Applications, and Technical Data
Part Numbers and Prices
Cross Referencing Agarose Products and Applications
Electro-Elution removes a 20-50x molar excess of fluorescent dyes from labelled proteins in 20 minutes
Gel Extractor, Protein Concentrator, Excess Dye or SDS Removal Device
Fast ElectroPrep for 20 minute
from Labelled Protein with Minimal Protein Loss.
Protein Gel Destaining
Protein Gels. Crystal clear gels, no blue desk tops, and less expensive
to use than tissues or foam. Zap those Coomassie Blues!
Part Numbers & Prices
Go back to .
Knitted Open Tubular (KOT) Reaction Delay & Mixing Coils
U.D.Neue, Geometrically deformed tubes as efficient vessels for post-column derivatization in high-performance
liquid chromatography,
KOT Reactors
KOT Reactor
Process Columns
Adjustable, Empty Glass . An overview of properties and capabilities.
Go back to .
If you have problems or comments concerning
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Last Updated:&11/18/14}