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您所在位置：&>>>&&&Dig-RNA labeling kit 1StRoche
Dig-RNA labeling kit 1StRoche
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【简单介绍】
Dig-RNA labeling kit 1StRoche作为全球最大的生物技术公司，罗氏在抗肿瘤、抗病毒、炎症、代谢和中枢神经系统，地高辛，细胞凋亡，细胞增殖等领域拥有一流的差异化药物。罗氏在全球体外诊断和基于组织的肿瘤诊断市场中享有领导地位，同时也是糖尿病管理领域的先驱者。
【详细说明】
罗氏总部位于瑞士巴塞尔，是一家以研发为基础、创新为驱动的全球医疗保健企业，拥有全球领先的制药和诊断业务。作为全球最大的生物技术公司，罗氏在抗肿瘤、抗病毒、炎症、代谢和中枢神经系统，地高辛，细胞凋亡，细胞增殖等领域拥有一流的差异化药物。罗氏在全球体外诊断和基于组织的肿瘤诊断市场中享有领导地位，同时也是糖尿病管理领域的先驱者。罗氏倡导的&个体化&医疗方案旨在通过创新的药物和诊断工具，显著改善人类的健康水平、生活质量以及患者的生存期Roche现货优惠促销&名称&&&&&& 品牌&&&&&& 货号&&&&&& 规格PVDF Western Blotting Membrane&&&&& Roche&&&& &&& 1 roll （30 cm x 3.00 m）Proteinase K,recomb.,PCR Grd.S&Roche&&&& &&& 1.25 mlGenopure Plasmid Midi Kit&& Roche&&&& &&& RT*1 kit （for up to 20 preparations）Genopure Plasmid Maxi Kit&Roche&&&& &&& RT*1 kit （for up to 10 preparations）Terminal Transferase, recomb.&&&&& Roche&&&& &&& 24,000 U （for 60 tailing or 3'-end labeling reactions）Protector RNase Inhibitor, 10&&&&&& Roche&&&& &&& 10,000 U （5 x 2,000 U） FRDIG-11-UTP,LSg.,3,5mM,200nmol&&&&& Roche&&&& &&& 200 nmol （3.5 mM, 57 ?l）Proteinase K recombinant PCR Grade&& Roche&&&& &&& 100mg （2&to 8&）cOmplete,EASYpack,20 Tab. Roche&&&& &&& 20 tablets （in foil blisters）cOmplete,EDTA-free,EASYpack,20&&&& Roche&&&& &&& 20 tablets （in foil blisters）cOmplete,Mini,EDTA-free,EASYpa&&&& Roche&&&& &&& 30 tablets （in foil blisters）LightCycler 480 SYBR Green I Master, 5 x 1 ml Roche&&&& &&& 5&100反应（20 &l） FRFastStart PCR Master 10 ml&Roche&&&& &&& 8 x 1.25 ml FRLightCycler 480 Multiwell Plate 96&&&&& Roche&&&& &&& 5 x 10 块板 （含封板膜）LightCycler 480 Multiwell Plate 384&&& Roche&&&& &&& 5 x 10 块板 （含封板膜）Cytotoxicity Det.Kit PLUS （LDH Roche&&&& &&& 1 kit （400 tests in 96 wells）*FRLightCycler 480 SYBR Green I Master, 10 x 5 ml&&&&& Roche&&&& &&& 10&500反应（20 &l）*FRTranscriptor cDNA Synth. Kit 1&&& Roche&&&& &&& 1 kit （100 reactions）*FRTranscriptor cDNA Synth. Kit 2&&& Roche&&&& &&& 1 kit （200 reactions）*FRPhosStop, 20 Tablets&&&&& Roche&&&& &&& 20 tablets （each tablet for 10 ml）PhosStop, 10 Tablets&&&&& Roche&&&& &&& 10 tablets （each tablet for 10 ml）FS Universal SYBR Green Master Roche&&&& &&& 4 x 1.25 ml （for 200 reactions of 50 ?l final reaction volume）FastStart Universal Probe Mast&&&& Roche&&&& &&& 2 x 1.25 ml （for 100 reactions of 50 ?l final reaction volume）FastStart Universal Probe Mast&&&& Roche&&&& &&& EALightCycler Capillaries （20 ?l）&&&&& Roche&&&& &&& 5x 96根/盒Dispase II （neutral protease,&Roche&&&& &&& 5 x 1 g CRCell Proliferation Reagent WST&&& Roche&&&& &&& 8 mlLightCycler 480 Multiwell Plate 384, clear&Roche&&&& &&& 5 x 10 块板 （含封板膜）E-Plate 16 （6x6 plates）&Roche&&&& &&& 6x6 plates RTE-Plate 16 （6 plates）&&&&& Roche&&&& &&& 6 platescOmplete ULTRA Tablets, Mini, EDTA-free, EASYpack&&& Roche&&&& &&& 30 tabletscOmplete ULTRA Tablets, Mini, EASYpack Roche&&&& &&& 30 tabletsX-Tremegene Hp Dna Transf.Reag.Trial&&&&& Roche&&&& &&& trial packX-tremeGENE 9 DNA Transf. Reag. 1.0 ml Roche&&&& &&& 1.0 ml*CRX-tremeGENE HP DNA Transf. Reag. 1.0 ml&&&& Roche&&&& &&& 1.0 mlDNase I, Grade II&& Roche&&&& &100 mgPronase from Streptomyces gris&&& Roche&&&& &1 g （non-sterile） CRDAPI&&&&& Roche&&&& &10 mg RTPolyethylene Glycol 1500 （PEG&& Roche&&&& &10 x 4 mlEndoproteinase Lys-C Seq. Grad&& Roche&&&& &3 x 5 ?g CRAnti-digoxigenin AP-conjungate&& Roche&&&& &150 U （200 ?l）Dig-RNA labeling kit 1St&&&&& Roche&&&& &1 kit （2 x 10 labeling reactions）Anti-digoxigenin-fluorescein Roche&&&& &200 ?gDIG-11-UTP&& Roche&&&& &250 nmol （10?mM, 25 ?l）Protein G-Agarose&Roche&&&& &5 mlDIG RNA Labeling Mixture, 10x&Roche&&&& &40 ?l （20 reactions）Mycoplasma Detection Kit&&& Roche&&&& &1 kit （25 tests）Nylon Membranes, 1 roll&&&&& Roche&&&& &1 roll （0.3 x 3 m）PCR DIG labeling Mix 1pc&& Roche&&&& &500 ?l （2 x 250 ?l） （for 2 x 25?reactions of 100 ?l final reaction volume）DIG High Prime Lab./Det.Kit II&& Roche&&&& &1 kit （12 labeling reactions and 24 detection reactions）DIG Wash and Block Buffer Set&& Roche&&&& &1 set （30 blots）DIG Easy-Hyb&&&&&& Roche&&&& &500 mlPCR Dig Probe Synthesis 1pc&&&&&& Roche&&&& &1 kit （25 reactions）Cell Proliferation Reagent WST&&& Roche&&&& &25 ml （2,500 tests）Cell Proliferation ELISA,BrdU&&&& Roche&&&& &1 kit （1,000 tests）TRIPURE ISOLATION REAGENT 200&&&&& Roche&&&& &200 mlNBT/BCIP Stock Solution&&&& Roche&&&& &8 mlIn Situ Cell Death fluorescein&&&&&& Roche&&&& &1 kit （50 tests）In Situ Cell Death POD Roche&&&& &1 kit （50 tests）Fluorescein RNA Label.Mix 10x&& Roche&&&& &40 ?l （20 reactions）Expand HiFi PCR Sys., 100 u&&&&& Roche&&&& &100 UExpand HiFi PCR Sys., 500 u&&&&& Roche&&&& &500 U （2 x 250 U）DIG High Prime Lab/Detection K Roche&&&& &1 kit （12 labeling reactions and 24 detection reactions）HP Plasmid Isolation Kit, 50 p&&&&& Roche&&&& &1 kit （50 purifications）Cell Death Detection ELISA^PLU&&&&&& Roche&&&& &1 kit （96 tests）HP PCR Template Preparation Ki&Roche&&&& &1 kit （100 purifications）MINI QUICK SPIN RNA COLUMNS&Roche&&&& &50 columnsAnti-GFP&&&&&& Roche&&&& &200 ?gHP RNA Isolation Kit&&& Roche&&&& &1 kit （50 reactions）b-Gal Staining Set&Roche&&&& &1 set （for 100 tests in 3.5-cm dishes）Annexin V FLUOS Staining Kit&& Roche&&&& &1 kit （50 tests）Anti-HA High Affinity （3F10）,&&&& Roche&&&& &50 ?g *CRCDP-Star, ready-to-use&Roche&&&& &2 x 50 mlIn Situ Cell Death TMR Roche&&&& &1 kit （50 tests）Anti-HA-Biotin, High Affinity&&&& Roche&&&& &50 ?g CR"Restr.-Endonucl. Sma I, 1000 U&"&&&&& Roche&&&& &1,000 U （10 U/Ul）"Restr.-Endonucl. Bam HI, 1000u "&&&&& Roche&&&& &1,000 U （10 U/Ul）"Aprotinin, 10mg&& "&&&&& Roche&&&& &10 mg DNA Ligase, T4, 100units&&&& Roche&&&& &100 U （1 U/Ul）"Restr.-Endonucl. Pst I, 3000 U&&& "&&&&& Roche&&&& &3,000 U （10 U/Ul）"Restr.-Endonucl. Hind III, 500U "&&&&& Roche&&&& "&&&&&& "&&&&& 5,000 U （10 U/&l）Restr.-Endonucl. Eco RV, 2000 && Roche&&&& &2,000 U （10 U/Ul）Restr.-Endonucl. Xba I, 1000 U&&& Roche&&&& &1,000 U （10 U/&l）"Restr.-Endonucl. Xho I, 5000 U&"&&&&& Roche&&&& &5,000 U （10 U/&l）"Anti-Digoxigenin-AP, Fab fragments&"&&&&& Roche&&&& &150 U （200ul）Blocking Reagent 50 g Roche&&&& &50 g Restr.-Endonucl. Eco RI&&&&&& Roche&&&& &10,000 U （10 U/Ul）DIG-11-UTP&& Roche&&&& &Collagenase P ROCHE&&100mg"DIG RNA Labeling Mixture, 10x &&&&& 40 ?l （20 reactions）"&&&&& Roche&&&& &Mycoplasma Detection Kit&ROCHE&&1 kit （25 tests）"BM Chem-Lum. WB-Substr. （POD） &"&&&&& Roche&&&& &1 set （4,000 cm2 membBM Chem-Lum. WB-Substr. （POD） && Roche&&&& &1 set （1,000 cm2 memb"DIG Wash and Block Buffer Set &&&&&& 1 set （30 blots）"&&&& Roche&&&& &1 set （30 blots）DIG Easy-Hyb 500 ml Roche&&&& &"Rapid DNA Ligation Kit&&&&& 1 kit"&&&&& Roche&&&& &40 DNA ligation reacti"Cytotoxicity Detection Kit （LD&& 1 kit"&&&&& Roche&&&& &（2,000 tests）"In Situ Cell Death fluorescein&&&& 1 kit （50 tests）"&&&&& Roche&&&& &1 kit （50 tests）"In Situ Cell Death POD&&&&&& 1 kit （50 tests） 5899"&&&&& Roche&&&& &1 kit （50 tests）"Fluorescein RNA Label.Mix 10x&"&&&&& Roche&&&& &40 Ul （20 reactions）Streptavidin, recombinant, 1 m&&&& Roche&&&& &1 mg Expand HiFi PCR Sys., 100 u&&&&& Roche&&&& &Expand HiFi PCR Sys., 500 u&&&&& Roche&&&& &500 U （2 x 250 U）"HP PCR Template Preparation Kit&&&&& "&&&&& ROCHE&&1 kit （100 purifications）Anti-GFP&&&&&& Roche&&&& &200 Ug HP RNA Isolation Kit&&& Roche&&&& &1 kit （50 reactions）"Titan One Tube RT-PCR System（1&&&&& "&&&&& Roche&&&& &100 reactions HP Viral Nucleic Acid Kit&&&& Roche&&&& &1 kit （for up to 100 purifFuGENE 6 Transf. Reag., 1 ml&&&& Roche&&&& -1ml&##1ml（300 transfections）FuGENE 6 Transf. Reag., 1 ml&&&& Roche&&&& -1ml&1ml（300 transfections）Lumi-Light^PLUS WB Substrate&Roche&&&& &100 ml （1,000 cm2 membr"CDP-Star, ready-to-use 2 x 50 ml "&&&& Roche&&&& &2 x 50 ml MagNA Pure LC Tip Stand &Roche&&&& "&"&&&&& 200 个Genopure Plasmid Maxi Kit&Roche&&&& &&& 1 kit （for up to 10 preparaProtector RNase Inhibitor, 10&&&&&& Roche&&&& -1ml&&& 2000 U*FRPwo SuperYield DNA Polymerase &&&&& Roche&&&& &&& 100 U siRNA Transfection Reagent&Roche&&&& -1ML&& 1MLFastStart TaqMan Probe Master&&&& Roche&&&& &&& 2 x 1.25 ml （for 100 rcOmplete,Mini,EASYpack,30 Tab.&&&&&& Roche&&&& &&& 30 tablets （in foil blisters）cOmplete,EDTA-free,EASYpack,20table&&&& Roche&&&& &&& FuGENE HD Transfection Reagent&&&&& Roche&&&& -1ml&&& ##1mlFastStart PCR Master 10 ml&Roche&&&& &&& 8 x 1.25 ml （for 400 react"DNase I recombinant, RNase-fre "&&&&& Roche&&&& &&& 10,000 U Complete Lysis, mammalian&Roche&&&& &&& 1 kit （200 ml lysis reagentComplete Lysis, mammalian EDTA&&&&& Roche&&&& &&& 1 kit 200 ml lysis reLightCycler 480 Multiwell Plate 96 &&& Roche&&&& &&& 5 x 10 块板LightCycler 480 Multiwell Plate 96&&&&& Roche&&&& -1pk&&& 1pkLightCycler 480 Multiwell Plate 384 &Roche&&&& &&& 5 x 10 块板LightCycler? 480 Multiwell Plate 384 white Roche&&&& -1plate ##1 plateRTLightCycler 480 Sealing Foil Roche&&&& -1pk&&& 1片"FastStart Taq DNA Pol. dNTPack&&&&&& "&&&&& Roche&&&& &&& 5,000 U （20 x 250 U）Expand HiFi PLUS PCR System dN&&& Roche&&&& &&& 500 U （2 x 250 U）PhosStop, 10 Tablets&&&&& Roche&&&& &&& 10 tablets （each tablet for 10 mFS Universal SYBR Green Master（Rox）&&&&& Roche&&&& -5ml&&& ##5ml/pk"FS Universal SYBR Green Master&&&&& "&&&&& Roche&&&& -5ML&& 5 ml （for 2,000 rDispase II （neutral protease&& Roche&&&& &&& 5 x 1 g Transcriptor HiFi cDNA Synth.&&& Roche&&&& &&& 1 kit （50 reactions, iTranscriptor HiFi cDNA Synth. && Roche&&&& &&& 1 kit （100 reactions）LightCycler 480 Multiwell Plate 384, clear&Roche&&&& -1pk&&& 1plate"cOmplete ULTRA Tablets, Mini, EDTA-free, EASYpack&& "&&&&& Roche&&&& &&& 30 tabletscOmplete ULTRA Tablets, Mini, EDTA-free, EA Roche&&&& -10tablets&&& 10tabletscOmplete ULTRA Tablets, Mini, EASYpack ROCHE&&&& 30 tabletsX-tremeGENE 9 DNA Transf. Reag.&&& Roche&&&& -1ml&&& ##1.0 ml CRX-tremeGENE 9 DNA Transf. Reag. &Roche&&&& -1ML&& 1.0 mlX-tremeGENE HP DNA Transf. Reag. Roche&&&& -1ml&&& ##1.0 ml*FR地高辛，细胞凋亡，细胞增殖
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产品详细描述
PCRSouthern5-10mgDNA, DNA
Application
The kit is specially designed for the generation of highly sensitive hybridization probes that are suitable for the detection of low- (single-) copy target sequences. Thus, it should be considered as an alternative to random-primed labeling when either the amount of template DNA is limited or only a specific part of the sequence of the template is required for hybridization. The nucleotide concentration in the PCR DIG Probe Synthesis Kit ensures the identification of single-copy genes in the genomic blots following hybridization to DIG-labeled PCR products. Human single-copy genes are detectable in 10 &g of genomic DNA.PCR products can be directly generated and labeled from small amounts of genomic DNA (100 ngC1 &g), and subsequently used as hybridization probes.The PCR DIG Probe Synthesis Kit contains an alkali-labile DIG-11-dUTP formulation. This enables simple removal of the DIG label following chemiluminescent detection, and allows the subsequent rehybridization of blots with multiple DIG-labeled probes.
Background Information
The nonradioactive DIG system uses digoxigenin, a steroid hapten, to label DNA, RNA, or oligonucleotides for hybridization, and subsequent color- or luminescent detection. The digoxigenin is coupled to dUTP via an alkali-labile ester bond. The labeled dUTP can be easily incorporated by enzymatic nucleic-acid synthesis using DNA polymerases.The polymerase chain reaction (PCR) allows the amplification of minute amounts of DNA to levels above 1 &g. The only prerequisite is that some sequence information of the target sequence is needed in order to synthesize the appropriate primers.The combination of nonradioactive labeling with PCR is a powerful tool for the analysis of PCR products, and also for the preparation of labeled probes from small amounts of a respective target sequence. The detection sensitivity of these labeled probes can be adjusted by the ratio of the unlabeled nucleotides to DIG-dUTP. Whereas a low ratio of DIG-dUTP to dTTP of 1:19, as in the PCR DIG Labeling Mix, ensures a high-yield amplification reaction with good sensitivity for direct detection, the hybridization to single-copy genes in complex genomes may not be successful. The PCR DIG Probe Synthesis Kit, therefore, contains a high ratio of DIG-dUTP to unlabeled nucleotides of 1:2, ensuring reliable detection of single-copy genes. However, PCR yield may be decreased by up to 50%. Nevertheless, this ratio will yield enough product for several hybridizations.
Enzyme Mix, Expand High Fidelity, 30 &l (105 U) Enzyme Mix, 3.5 U/&l in storage buffer, 20 mM Tris-HCl, pH 7.5 (25oC), 100 mM KCl, 1 mM dithiothreitol (DDT), 0.1 mM EDTA, 0.5% Tween 20 (v/v), 0.5% Nonidet P40 (v/v), 50% glycerol (v/v)
PCR DIG Probe Synthesis Mix, 10x conc., 125 &l of a mixture containing dATP, dCTP, dGTP (2 mM each), 1.3 mM dTTP, 0.7 mM DIG-11-dUTP, alkali-labile, pH 7.0
PCR Buffer with MgCl2, 10x conc., 1 ml Expand High Fidelity Buffer, 10x conc. with MgCl2
dNTP Stock Solution, 10x conc., 125 &l of a mixture containing dATP, dCTP, dGTP, dTTP (2 mM each), pH 7.0
Control Template, 50 &l (1 ng) plasmid DNA (20 pg/&l) in Tris/EDTA buffer, pH 8.0. The 5-kb plasmid contains the cDNA for the human tissue-type plasminogen activator (tPA)
Control PCR Primer Mix, 25 &l (containing 50 pmol of each primer) control PCR primer 1 and 2 (2 mM each)
Function test: Each lot of the PCR DIG Probe Synthesis Kit is function-tested in PCR. Amplification products are assayed in genomic Southern blots.Under PCR conditions described in the pack insert, the control reaction generates an amplification product of 442 bp. Due to multiple incorporations of DIG-dUTP during the PCR process, the molecular weight of the PCR products is significantly increased compared to the unlabeled PCR product.A specific fragment pattern is detected after hybridization of the PCR product to 10 &g human genomic DNA and chemiluminescent detection as described in the pack insert.
DIG-11-dUTPDNA, (AP), SouthernNorthern: DNA1; DNA2; DNA; DIG-DNA; ; Klenow 2u/μl; ; BCIP/NBT; 10×dNTP; --Kit2550(100cm2), -20℃
DIG-High Prime DNA Labeling and
Detection Starter Kit I
DIG, , SouthernNorthernKit1224: 5×DIG-; DNA, PBR328; DNA; -DIG-; NBT/BCIP; 10×; DIG; DIG, -20℃
Detection Starter Kit II
DIG, CSPDKit1224: 5×DIG; DNA, PBR328; DNA; -DIG-; CSPD; 10×; DIG; DIG, NBT/BCIP; -20℃
DIG DNA Labeling Kit
DNA标记试剂盒
DIG-11-dUTPDNA; DIG
DIG Nucleic acid Detection Kit
NBT/BCIP, 0.1pgDNA; 40100cm2-20℃
DIG Oligonucleotide 3’-End Labeling Kit
DIG-11-ddUTP14-100bp3’100pmol
DIG Oligonucleotide Tailing Kit
DIG-11-ddUTP3’50bp(10-100bp), DNA/RNA100pmol
DIG RNA Labeling Kit
RNA(SP6/T7)
SP6T7 RNARNA, 1mg10mgRNA-20℃: pSPT18 DNA, pSPT19 DNA, DNA1, DNA2, DIGRNA, NTR, , RNase, RNaseDNase I, SP6 RNA, T7 RNA
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