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基于基因芯片数据初步分析绒毡层转录调控通路
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3秒自动关闭窗口武汉植物园揭示水稻种子萌发过程的分子调控机制----中国科学院
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武汉植物园揭示水稻种子萌发过程的分子调控机制
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　　水稻种子萌发是其成长为独立生命个体重要的起始点，是形成良好的幼苗的前提条件。水稻种子包含胚、胚乳等两大主要组织，在萌发过程中，胚组织的营养物质逐步分解并运输至胚中，为胚发育形成幼苗提供必要的物质和能量条件。胚组织虽然体积较小，但在萌发过程中却处于中心地位。因此，有必要对水稻胚和胚乳在萌发过程中的不同作用进行深入研究，从而深入探讨水稻种子萌发过程的分子调控机制。　　中国科学院武汉植物园资源植物繁殖生物学课题组博士研究生韩超在杨平仿研究员指导下，利用基于凝胶的双向电泳技术和非凝胶的i-TRAQ技术，对水稻种子萌发过程中胚和胚乳组织分别进行蛋白质组研究。共获得343个胚组织蛋白质在萌发过程中的表达量发生改变，包括191个下调表达和152个上调表达。这些蛋白质参与众多能量代谢途径，其中包括参与淀粉合成、支链淀粉合成的酶、淀粉粒合成酶等在萌发早期阶段上调表达而在后期阶段下调表达，该结果与实验室前期研究结果发现水稻种子萌发过程中胚组织发现淀粉粒一致。胚组织中的蔗糖可能作为中间物为淀粉合成提供帮助。此外，通过该研究发现在水稻种子萌发过程中，胚乳组织的大量生物学过程是受胚组织调控的。　　以上研究结果得到国家自然基金（）、中国科学院“百人计划”和中非联合研究项目（SAJC201324）的经费支持。相关结果发表于国际期刊Plant and Cell Physiology。　　0利用MAPMAN分析水稻种子萌发过程中的代谢途径
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地址：北京市三里河路52号 邮编：100864植物学报（英文版）060513
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
2006 Vol.48 No.5 P.591-612
Transcriptional Responses to Gibberellin and Abscisic Acid in Barley Aleurone
Kegui Chen （Department of Agronomy, University of Wisconsin, Madison, WI 53706, USA）　
Yong-Qiang Charles An（United States Department of Agriculture, Agricultural Research Service, Cereal Crops Research, Madison, WI 53726, USA）　
Abstract：Cereal aleurone has been established as a model system to investigate giberrellin (GA) and abscisic acid (ABA) responses. Using Barley 1 GeneChip, we examined the mRNA accumulation of over 22 000 genes in de-embryonated barley aleurone treated with GA and ABA. We observed that 1328 genes had more than a threefold change in response to GA treatment, whereas 206 genes had a more than threefold change in response to ABA treatment. Interestingly, approximately 2.5-fold more genes were up-regulated than downregulated by ABA. Eighty-three genes were differentially regulated by both GA and ABA. Most of the genes were subject to antagonistic regulation by ABA and GA, particularly for genes related to seed maturation and germination, such as genes encoding late embryogenesis abundant proteins and storage mobilization enzymes. This supports the antagonistic roles of GA and ABA in seed maturation and seed germination.Interestingly, we observed that a significant percentage of the genes were coordinately regulated by both GA and ABA. Some GA-responsive genes encoded proteins involved in ethylene, jasmonate, brassinosteroid and auxin metabolic and signaling transduction pathways, suggesting their potential interaction with the GA response. We also identified a group of transcription factor genes, such as MYB and Homeobox genes, that were differentially regulated by GA. In addition, a number of GA- and/or ABA-responsive genes encoded components potentially involved in GA and ABA signal transduction pathway. Overall, the present study provides a comprehensive and global view of transcript expression accompanying the GA and ABA response in barley aleurone and identifies a group of genes with potential regulatory functions in GA- and ABA-signaling pathways for future functional validation.
Keywords： signal transduction.
Foundation Item：Supported by the US Department of Agriculture, Agricultural Research Service and the US Barley Genome Project.Publication of this paper is supported by the National Natural Science Foundation of China () and Science Publication Foundation of the Chinese Academy of Sciences.
Biography：Yong-Qiang Charles An ,Author for correspondence. Tel: +1 608 358 8162; Fax: +1 608 890 0302; E-mail: .
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